DuelingSyringes™ Tissue Disperser
A low cost, disposable, tissue disperser for up to 100 mg samples of soft tissue (e.g., liver, brain and muscle). The Dueling Syringes™ tissue disperser is a significant improvement over the classic "syringe-needle-syringe" method of tissue dispersion. The needle is replaced with an embedded 500 micron stainless steel screen inside a 1 ml polycarbonate syringe. Manual passage of the sample back and forth through the screen is fast, gentle and efficient. Sold as a pack of five pairs of syringes.
Our Price : $0.00
The DuelingSyringes™ tissue disperser is a low shear method used to recover intact, viable cells or as an initial step in the isolation of high quality intracellular organelles in a native condition. The disperser consists of a pair of 1 ml syringes connected together via their Luer connections. One of the syringes has an embedded 500 micron stainless steel screen. Tissue is loaded into the syringe and forced back and forth through the screen by alternate manual pushing of the syringe plungers. Compared to the classic syringe-needle-syringe method of tissue dispersion, the DuelingSyringes method is faster and more gentle in the processing of small samples of soft tissue. Less pushing effort is required and the number of multiple passes from one syringe to the other is decreased. Glass and metal syringes used in the classic method are replaced with low cost, disposable syringes...thus, cross contamination and clean-up concerns are eliminated.
For details on the operation of the DuelingSyringes™ tissue disperser see Operating Instructions.
- Gentle, low shear, dispersion of soft animal tissue. Cannot be used with skin, cartilage, or other tough, fibrous tissues.
- Low cost, manuel method.
- Conveniently disposable.
- Improved through-put.
- Ideal for small sample sizes (<100 mg="" wet="">
You May Also Be Interested In...
Screw Tissue Press
The Screw Tissue Press is used as an initial tissue dispersion step before lysing fresh tissue1 with a mechanical homogenizer (ultrasonicator, beadbeater, rotor-stator, etc) or the preparation of single cells. Chopping tissue into pieces with scissors or scalpel is eliminated, and much smaller tissue fragments are produced. Unlike other tissue dispersion/blending devices, no buffer or media is added during its operation. The dispersed tissue exits out of the end of the Screw Tissue Press has a consistency much like liver paté or toothpaste.
The Screw Press also is used to prepare viable single cells, either directly2 or as a first step via enzyme digestion of the extracellular connective matrix. The benefits of an initial Screw Press disaggregation of tissue is reduced incubation times with the digestive enzymes and increased diffusion of critical nutrients and gases into tissue cells during the incubation period. The net result is higher yields of viable cells. [ For an excellent general review see Methods of Enzymatic Tissue Disaggregation ].
1. Tissue processed in this press must be "soft" (i.e., relatively non-fibrous and have high water content). Acceptable examples are muscle, brain, liver, plant fruits, some plant roots and tubers.
2. Example:>A rapid method for the preparation of single‐cell suspensions from solid tissues, NP Singh - Cytometry 31: 229–232, 1998
Read more >Price: $36.00
MicroMincer
Cat. No. 2941, MicroMincer laboratory press complete with a 10-pack of liner and sealsCat. No. 294103, Extra liners and seals, 10 of each
Read more >
