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商品详细biospec/BioSqueezer/1kit/1210
biospec/BioSqueezer/1kit/1210
biospec/BioSqueezer/1kit/1210
商品编号: 1210
品牌: biospec
市场价: ¥3520.00
美元价: 1760.00
产地: 美国(厂家直采)
公司:
产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

BioSqueezer

The BioSqueezer is used to "snap-freeze" (quickly freeze) 10-100 mg of soft animal and human tissue. It does the same thing as a classic freeze clamp but is easier to use and gives more reproducable results.

Fresh tissue is compressed into a thin disc about the size of a small coin under pressure from a stainless steel clamp head that has been pre-cooled in liquid nitrogen or dry ice.Freezing occurs in a few milliseconds, thus offering a "snapshot" of metabolic events in the cells. The hard frozen disc is easily fragmented by hand or by grinding in a Cryo-Cup mortar and pestle for subsequent rapid extraction of labile nucleic acids*, proteins and other intracellular products.

* Very high molecular weight DNA is obtained by cryo-pulverizing hard frozen tissue using one of our dry-milling devices (see Cryo-Cup mortar and pestle, BioPulverizer , or MiniBeadbeater) followed by passive extraction (i.e., minimum physical stirring, pipetting or agitation during the cell lysis step in DNA extraction solution).

Our Price : $0.00

How the BioSqueezer is operated...

A small beaker, styrofoam cup, or similar vessel is placed on the BioSqueezer platform and the metal clamp is lowered.  Liquid nitrogen is slowly and carefully poured into the vessel until the boiling action of the liquid nitrogen subsides.  The cooling process takes about a minute and, once cooled to the desired temperature, the stainless metal clamp head will remain adequately cold for several minutes.  The vessel is removed and the fresh sample, by itself or already placed in a sterile poly-bag*, is placed on the platform.  The s.s. head is quickly lowered and clamped.  Clamping must be done rapidly or the sample will freeze uncompressed.It may be necessary to adjust the BioSqueezer using 'practice' tissue.  The optimal clamp setting can vary with the softness and size of the tissue sample.  Clamping force and the degree of squeezing on the tissue can be easily adjusted by changing the position of the metal clamp head with the wing nut, black plastic nut and stainless steel hex bolt.The thin wafer of tissue can be manually fragmented inside the poly bag while still hard frozen.  Fragmenting hard frozen tissue into small pieces speeds up subsequent chemical extraction or mechanical homogenization and also inhances the penetration of RNAlater and other expediants used for tissue preservation and stabilization of intracellular biochemicals.

*  A sample pack of Poly-Bags is included with the BioSqueezer.

品牌介绍
美国Biospec公司从事科学仪器近三十年,为许多实验室提供了高新的仪器设备,在组织研磨方面更是处于**地位。无泡沫,不产生气体,可以保持酶的活性,保护核酸和细胞器官,是进行PAGE,聚合酶实验和利用抗体或者寡核苷酸探针诊断的*佳仪器。这使我们能够以优惠的价格提供高品质的产品。