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当前位置: 首页 > 产品中心 > Targeted_sequencing_kit > biospec/不锈钢微孔-2 ml/1/2007
商品详细biospec/不锈钢微孔-2 ml/1/2007
biospec/不锈钢微孔-2 ml/1/2007
biospec/不锈钢微孔-2 ml/1/2007
商品编号: 2007
品牌: biospec
市场价: ¥1740.00
美元价: 870.00
产地: 美国(厂家直采)
公司:
产品分类: 靶向测序试剂盒
公司分类: Targeted_sequencing_kit
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

Stainless Steel Microvials - 2 ml

Cat. No. 2007, 1.8 ml capacity type 305 stainless steel microvials. Polyethylene flange caps are included1. Package size:100 pieces

Our Price : $0.00

Our Stainless Steel Microvials have the same physical dimensions and weight as standard 2 ml polypropylene microvials. They can be sealed with either polyethylene flange caps, silicone rubber stopper caps1 or crimped 13 mm OD septa caps.

Applications include:

  • Dry grinding with steel beads in a high energy Bead mill cell disrupter (e.g.,MiniBeadbeater) where milling forces can crack common plastic microvials - - an exception is our 2 ml XXTuff polypropylene microvials.   They are the toughest 2 ml screw-top polypropylene vial on the market and can be used for most dry milling applications).
  • Provides far superior heat transfer capabilities compared to plastic or glass vessels - as might be desired for some PCR methods and the control of sample heating during the beadbeating  process.

 1  NOTE:  When beadbeating dry or hard frozen samples using steel beads Silicone Rubber Caps, specially shaped to seal our s.s. microvials, should be used. Flange caps can fail under these milling conditions.  Scroll down for more information on Silicone Rubber Caps.

 

A protocol for dry grinding small frozen samples at liquid nitrogen temperature (i.e., cryo-grinding): Three 3.2 mm dia. chrome-steel beads are added to a 2 ml stainless steel vial. The vial and beads are brought to liquid nitrogen temperature...without entrapping any liquid nitrogen inside the vial. Then add hard frozen cartilage, skin or other tough tissue (pre-chopping the sample is usually not necessary)*, quickly seal the vial with a non-frozen silicone rubber cap and grind for 5-15 seconds in a MiniBeadbeater. The sample will be powdered.If extraction of intracellular biomolecules is to follow, promptly add ice-cold liquid media of your choice to the still frozen, powdered sample in the original vial and beadbeat for up to 3 minutes. The optimal beadbeating time will vary with sample type and should be empirically determined with an initial time study of 0.5, 1, 2, and 3 minutes of beadbeating.

   *  Add pre-frozen tissue.  Fresh tissue will "stick" to the cold steel beads and delay good grinding.

品牌介绍
美国Biospec公司从事科学仪器近三十年,为许多实验室提供了高新的仪器设备,在组织研磨方面更是处于**地位。无泡沫,不产生气体,可以保持酶的活性,保护核酸和细胞器官,是进行PAGE,聚合酶实验和利用抗体或者寡核苷酸探针诊断的*佳仪器。这使我们能够以优惠的价格提供高品质的产品。