Lever Tissue Press
The Lever Tissue Press is a top-of-the-line stainless steel garlic press improved in performance by the addition of a silicone-rubber gasket on the lever press piston.The press efficiently macerates or mechanically disaggregates 5 to 50 grams of fresh, soft plant or animal tissue. By 'soft' we mean nonfibrous tissue. Acceptible examples are muscle, brain, liver, plant fruits, some plant roots and tubers. An extra "can" is included to facilitate processing of multiple samples.
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The Lever Tissue Press prepares tissue as an initial mincing step preceeding mechanical homogenization (cell disruption) or preceeding single cells isolation from solid tissue by digestion of extracellular connective matrix with proteolytic enzyme. The press eliminates tedious chopping of tissue into little pieces with a scissors or scalpel and produces much smaller tissue pieces. Unlike many other tissue dispersion/blending devices, no buffer is added in its operation.
Special App! Disaggregation of tissue for primary culture. - Incubation time needed for enzymatic tissue disaggregation is reduced and during the incubation period with the digestive enzymes, diffusion of nutrients and gases into the tissue mass are optimized. Expect higher yields of viable cells. For an excellent review of the Method of Enzymatic Disaggregation see http://www.worthington-biochem.com/tissuedissociation/default.html) .
Tissue processed in this press must be "soft", relatively non-fibrous and have high water content. Acceptible examples are muscle, brain, liver, plant fruits, and some plant roots and tubers. If the tissue is fatty or partially fibrous, manually trim off the tough or fatty parts before pressing. Fibrous tissue will interfere with the passage of the soft tissue through the press and lower the yield of dispersed cell material. Unacceptible tissues are skin, hide, connective tissue, woody or fibrous plant stems, many mature seeds and all dried tissues.
Operating Instructions:
1) Retract the piston of the Lever Tissue Press from its stainless steel "can". Add 1 to 10 g of fresh tissue into the cavity of the "can" and insert the piston back into position.
2) Gripping the two levers, force the piston down the can. Scrape the dispersed tissue protruding from the bottom of the can with a knife or scapel. Depending on the amount of connective or fibrous tissue present in the sample, the yield of dispersed tissue will vary from 20-95%.
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Screw Tissue Press
The Screw Tissue Press is used as an initial tissue dispersion step before lysing fresh tissue1 with a mechanical homogenizer (ultrasonicator, beadbeater, rotor-stator, etc) or the preparation of single cells. Chopping tissue into pieces with scissors or scalpel is eliminated, and much smaller tissue fragments are produced. Unlike other tissue dispersion/blending devices, no buffer or media is added during its operation. The dispersed tissue exits out of the end of the Screw Tissue Press has a consistency much like liver paté or toothpaste.
The Screw Press also is used to prepare viable single cells, either directly2 or as a first step via enzyme digestion of the extracellular connective matrix. The benefits of an initial Screw Press disaggregation of tissue is reduced incubation times with the digestive enzymes and increased diffusion of critical nutrients and gases into tissue cells during the incubation period. The net result is higher yields of viable cells. [ For an excellent general review see Methods of Enzymatic Tissue Disaggregation ].
1. Tissue processed in this press must be "soft" (i.e., relatively non-fibrous and have high water content). Acceptable examples are muscle, brain, liver, plant fruits, some plant roots and tubers.
2. Example:>A rapid method for the preparation of single‐cell suspensions from solid tissues, NP Singh - Cytometry 31: 229–232, 1998
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DuelingSyringes™ Tissue Disperser
A low cost, disposable, tissue disperser for up to 100 mg samples of soft tissue (e.g., liver, brain and muscle). The Dueling Syringes™ tissue disperser is a significant improvement over the classic "syringe-needle-syringe" method of tissue dispersion. The needle is replaced with an embedded 500 micron stainless steel screen inside a 1 ml polycarbonate syringe. Manual passage of the sample back and forth through the screen is fast, gentle and efficient. Sold as a pack of five pairs of syringes.
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